Molecular characterization of Malassezia species isolated from Pityriasis Versicolor patients in Thi-Qar Governorate / Iraq
DOI:
https://doi.org/10.66344/jpad.v36i1.2961Keywords:
Keywords : Malassezia species; 26S rDNA gene; β-tubulin gene; genetic diversity.Abstract
Abstract Background: Malassezia are lipophilic yeasts that coexist symbiotically on the skin of humans and other warm-blooded animals. They assume two forms: unicellular yeasts and pseudofilamentous yeasts. The Pityriasis versicolor is induced by Malassezia yeast. The yeasts invade the outermost keratinized layer of human skin and induce several alterations in this layer, such as the formation of different-colored patches on the skin. Objective: Comparison among the prevalence of Malassezia species in healthy and infected human using Molecular methods. Methods: About 72 skin scraping samples were collected from patients diagnosed by the dermatologist at AL-Nasiriyah General Hospital, AL-Hussein Teaching Hospital, and AL-Shatra General Hospital as pityriasis versicolor patients and 30 skin swabs from healthy individuals at the period from Jul-2023 to Dec-2023. Results: The results of the genetic sequence analysis of the PCR products of Malassezia isolates showed a large genetic diversity in the isolates of patients and healthy (control). The results showed the presence of several mutations in the DNA sequence of Malassezia isolates, and new strains were registered in the NCBI GenBank website. Conclusion: There is a genetic relationship between the pathogenic isolates and the control isolates and there is a high genetic diversity.References
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