Expression of MicroRNA-224-3p in Vitiligo

Abstract

Background : Vitiligo is a skin and less common hair disease characterized by decline melanocytes function and depigmentation with a prevalence of 0.5–1% in most populations. MicroRNAs (miRNAs) are small single stranded; approximately 22 nucleotide-long, non-coding gene-regulatory RNA molecules that inhibit gene expression through translational repression or mRNA turnover .Aim of our study :This case control study aimed at detection of the expression of microRNA-224-3p in lesional skin of vitiligo patients as compared to normal control persons to detect the possible role of microRNA-224-3p in the pathogenesis of vitiligo Material and subjects: This study included 60 patients with vitiligo and 60 age,gender matched healthy controls. 4 mm punch skin biopsy had been taken from patients( vitiligo lesion) & controls and it had been kept in lysis solution for the stability of the studied parameters and was kept frozen at -80 Celsius till analysis of microRNA-224-3p  by qRT PCR. Results:The level of microRNA-224-3p  in lesional skin of vitiligo was significantly higher as compared to normal control persons. Keywords : Vitiligo;MicroRNA-224-3p, polymerase chain reaction