Expression of MicroRNA-224-3p in Vitiligo

Authors

  • Rehab mohamed naguib mohamed naguib lecturer of dermatology,Beni suef university
  • Abd-El Aziz El-Rifaie Professor of dermatology, Dermatology & Venerology department, Faculty of Medicine, Beni-Suef University,
  • Laila Ahmed Rashed professor of Biochemistry , Cairo University .
  • Hanan Ahmed Ibrahim Dermatology department, Beni-Suef general Hospital

DOI:

https://doi.org/10.66344/jpad.31.4.2021.1788

Abstract

Background : Vitiligo is a skin and less common hair disease characterized by decline melanocytes function and depigmentation with a prevalence of 0.5–1% in most populations. MicroRNAs (miRNAs) are small single stranded; approximately 22 nucleotide-long, non-coding gene-regulatory RNA molecules that inhibit gene expression through translational repression or mRNA turnover .Aim of our study :This case control study aimed at detection of the expression of microRNA-224-3p in lesional skin of vitiligo patients as compared to normal control persons to detect the possible role of microRNA-224-3p in the pathogenesis of vitiligo Material and subjects: This study included 60 patients with vitiligo and 60 age,gender matched healthy controls. 4 mm punch skin biopsy had been taken from patients( vitiligo lesion) & controls and it had been kept in lysis solution for the stability of the studied parameters and was kept frozen at -80 Celsius till analysis of microRNA-224-3p  by qRT PCR. Results:The level of microRNA-224-3p  in lesional skin of vitiligo was significantly higher as compared to normal control persons. Keywords : Vitiligo;MicroRNA-224-3p, polymerase chain reaction  

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Published

2022-03-29

How to Cite

1.
mohamed naguib R mohamed naguib, Abd-El Aziz El-Rifaie, Laila Ahmed Rashed, Hanan Ahmed Ibrahim. Expression of MicroRNA-224-3p in Vitiligo. J Pak Assoc Dermatol [Internet]. 2022Mar.29 [cited 2026May3];31(4):672-6. Available from: https://www.jpad.com.pk/index.php/jpad/article/view/1788

Issue

Vol. 31 No. 4 (2021): October - December

Section

Original Articles

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