Expression of MicroRNA-224-3p in Vitiligo
DOI:
https://doi.org/10.66344/jpad.v31i4.1788Abstract
Background Vitiligo is a skin and less common hair disease characterized by decline melanocytes function and depigmentation with a prevalence of 0.5–1% in most populations. MicroRNAs (miRNAs) are small single stranded; approximately 22 nucleotide-long, non-coding gene-regulatory RNA molecules that inhibit gene expression through translational repression or mRNA turnover.
Objective This case control study aimed at detection of the expression of microRNA-224-3p in lesional skin of vitiligo patients as compared to normal control persons to detect the possible role of microRNA-224-3p in the pathogenesis of vitiligo.
Methods This study included 60 patients with vitiligo and 60 age, gender matched healthy controls. 4 mm punch skin biopsy had been taken from patients (vitiligo lesion) & controls and it had been kept in lysis solution for the stability of the studied parameters and was kept frozen at -80 Celsius till analysis of microRNA-224-3p by qRT PCR.
Results The level of microRNA-224-3p in lesional skin of vitiligo was significantly higher as compared to normal control persons.
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Copyright (c) 2022 Rehab mohamed naguib mohamed naguib, Abd-El Aziz El-Rifaie, Laila Ahmed Rashed, Hanan Ahmed Ibrahim
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