Role of autophagy related Gene 16 L1 in psoriasis pathogenesis
DOI:
https://doi.org/10.66344/jpad.v31i4.1762Abstract
Background Psoriasis is a chronic hyperproliferative inflammatory disease, in which genetic and environmental factors have an important role, but the exact cause is yet unknown . Autophagy is a strictly regulated lysosomal degradation pathway that is crucial for maintaining intracellular homeostasis and normal development. The dysregulation of autophagy-associated genes was recognized to increase the susceptibility to multiple diseases, including inflammation, autoimmune disorders, and cancer.
Objective Our study aimed to detect the expression of autophagy related gene 16 L 1(ATG 16L) in psoriasis patients compared to normal control persons to investigate the possible role of autophagy in pathogenesis of this disease.
Methods This case - control study included thirty psoriasis patients and thirty healthy controls .Four mm punch skin biopsies were obtained from psoriasis lesions and from the controls and they were kept in lysis solution for the stability of the studied parameters and were kept frozen at -80 Celsius till analysis of ATG 16L using real time polymerase chain reaction.
Results The level of autophagy related gene 16 L1 in lesional skin of psoriasis was significantly increased compared to normal control persons (p<0.001).
Conclusion Autophagy may play a role in the pathogenesis of psoriasis disease.
Limitation Limited number of patients included in our study (30 patients).
References
1. Parisi, R., Symmons, D.P., Griffiths, C.E. and Ashcroft, D.M. Global epidemiology of psoriasis: a systematic review of incidence and prevalence. J Invest Dermatol.2013; 133(2):377–385.
2. Raychaudhuri, S.K., Maverakis, E. and Raychaudhuri, S.P. Diagnosis and classification of psoriasis. Autoimmune Rev.2014;13(4-5):490–5.
3. Pierdominici M, Vomero M, Barbati C, et al. Role of autophagy in immunity and autoimmunity, with a special focus on systemic lupus erythematosus. FASEB J.2012;26(4):1400-12.
4. Douroudis, 4-K., Kingo, K., Traks, T., Rätsep, R., Silm, H., Vasar, E., & Kõks, S. (2011). ATG16L1 gene polymorphisms are associated with palmoplantar pustulosis. Hum Immunol.2011;72(7):613-5.
5. Liang, Y., Sarkar, M. K., Tsoi, L. C., & Gudjonsson, J. E. Psoriasis: a mixed autoimmune and autoinflammatory disease. Current Opin Immunol.2017;49:1-8.
6. Harden, J. L., Krueger, J. G., & Bowcock, A. M. The immunogenetics of psoriasis: a comprehensive review. J Autoimmun.2015; 64:66-73.
7. Greb JE, Goldminz AM, Elder JT, Lebwohl MG, Gladman DD, JJ W, Mehta NN, Finlay AY, Gottlieb AB. Psoriasis. Nat Rev Dis Primers.2016;2:16082
8. Guo, Y., Zhang, X., Wu, T., Hu, X., Su, J., & Chen, X. (2019). Autophagy in skin diseases. Dermatology.2019;235(5):380-9.
9. Lee, H. M., Shin, D. M., Yuk, J. M., Shi, G., Choi, D. K., Lee, S. H., & Jo, E. K. Autophagy negatively regulates keratinocyte inflammatory responses via scaffolding protein p62/SQSTM1. J Immunol.2011; 186(2):1248-58.
Downloads
Published
Issue
Section
License
Copyright (c) 2022 Rehab mohamed naguib mohamed naguib, Abd-El Aziz El-Rifaie, Eman Abdel Zaher Eissa, Laila Ahmed Rashed
This work is licensed under a Creative Commons Attribution 4.0 International License.
Submission declaration
Authors retain the copyright to their work and grant the 'Journal of Pakistan Association of Dermatologists (JPAD)' the right of first publication under a Creative Commons Attribution 4.0 International (CC BY 4.0) license. This license allows others to share, adapt, and reuse the work for any purpose, including commercial use, as long as appropriate credit is given to the original authors and the journal.
By submitting a manuscript, authors confirm that the work has not been published previously (except as an abstract, lecture, or academic thesis), is not under review elsewhere, and has been approved by all authors and relevant authorities. Once accepted, the article will be openly accessible under the CC BY 4.0 license, ensuring wide dissemination and reuse with proper attribution.
