Role of autophagy related Gene 16 L1 in psoriasis pathogenesis

Abstract

Background  Psoriasis is a chronic hyperproliferative inflammatory disease, in which genetic and environmental factors have an important  role, but the exact cause is yet unknown . Autophagy is a strictly regulated lysosomal degradation pathway that is crucial for maintaining intracellular homeostasis and normal development. The dysregulation of autophagy-associated genes was recognized to increase the susceptibility to multiple diseases, including inflammation, autoimmune disorders, and cancer.   Objective Our study aimed to detect the expression of autophagy related gene 16 L 1(ATG 16L) in psoriasis patients compared to normal control persons to investigate the possible role of autophagy in pathogenesis of this disease.   Methods This case - control study included thirty psoriasis patients and thirty  healthy controls  .Four mm punch  skin  biopsies  were obtained   from  psoriasis  lesions and from the controls  and  they were kept in  lysis  solution  for  the  stability  of the  studied  parameters and were  kept frozen at -80 Celsius till analysis  of ATG 16L   using real time polymerase chain reaction.   Results  The level of autophagy related gene 16 L1 in lesional skin of psoriasis was significantly increased compared to normal control persons (p<0.001).