Basil Leaf Extract and Eugenol against Isolates of Candida sp. Causing Oral Candidiasis in HIV/AIDS
DOI:
https://doi.org/10.66344/jpad.32.4.2022.2029Abstract
Background: Oral candidiasis is an opportunistic infection of the oral mucosa caused by Candida sp., frequently found in HIV/AIDS patients. Basil leaf extract (Ocimum sanctum Linn.), which contains eugenol, a compound believed to inhibit the growth of Candida sp.
Objective: To evaluate the antifungal susceptibility of basil leaf extract (Ocimum sanctum Linn.) and eugenol and then compared to fluconazole against Candida sp. isolates.
Method: Basil leaf extract (Ocimum sanctum Linn.) with doses equivalent to eugenol 800 µg/mL and 400 µg/mL, eugenol doses at 800 µg/mL and 400 µg/mL, which was then compared with fluconazole 25 µg/mL against 40 Candida sp. isolated stored from the oral cavity of HIV/AIDS patients.
Results: The average inhibition zone of fluconazole was 21.81 mm, the mean inhibition zone of eugenol with doses of 800 µg/mL and 400 µg/mL were 17.07 mm and 15.89 mm, and the mean inhibition zone of basil leaf extract (Ocimum sanctum Linn.) with doses equivalent to eugenol 800 µg/mL and 400 µg/mL were 14,87 mm and 14,01 mm (p = 0.001 and p < 0.05).
Conclusion: Fluconazole had a significantly higher inhibition zone against Candida albicans and Candida non-albicans isolates than basil leaf extract (Ocimum sanctum Linn.) and eugenol.
Keyword Basil leaf extract (Ocimum sanctum Linn.), eugenol, fluconazole, antifungal susceptibility testing, oral candidiasis, HIV, AIDS
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Copyright (c) 2022 Dwi Murtiastutik, Emma Hidayati Sasmito, Diah Mira Indramaya, Sawitri Sawitri, Rahmadewi Rahmadewi, Afif Nurul Hidayati, Pepy Dwi Endraswari, Sudjarwo Sudjarwo, Budi Utomo
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