Study of direct immunofluorescence in various autoimmune vesiculobullous disorders: an observational study
Keywords:
Direct immunofluorescence, immunobullous diseases, histopathology, sensitivity, diagnosisAbstract
Objectives The aim of the study was to see the clinical, histological and immunological correlation of various immunobullous diseases and to evaluate the sensitivity of direct immunofluorescence (DIF) in specific diagnosis of various immunobullous diseases. Methods 29 patients of immunobullous diseases attending skin OPD and IPD of a tertiary care hospital in Ujjain were included in the study and were thoroughly examined. Results Out of 29, 26 cases showed DIF patterns concordant with histologic diagnosis. The sensitivity of DIF was 92.3% (12/13) in the pemphigus group, 75% (3/4) in bullous pemphigoid, 66.7% (2/3) in dermatitis herpetiformis, while it was 100% (3/3) each in linear IgA disease and chronic bullous dermatosis of childhood. Conclusion DIF is essential for diagnosing autoimmune blistering diseases with the clinical and the histopathological overlap.ÂReferences
Wojnarowska F, Venning VA. Immunobullous diseases. In: Burns T,Breathnach S, Cox N, Griffiths C, editors. Rook’s Textbook of Dermatology. 8th ed. Oxford: Blackwell; 2010. p. 1 62
Huilgol SC, Bhogal BS, Black MM.“Immunofluorescence of immunobullous disorders part one: methodologyâ€, Indian J Dermatol Venereol Leprol. 1995; 61: 187-95.
Inchara YK, Rajalakshmi T. “Direct immunofluorescence in cutaneous vesiculobullous lesionsâ€, Indian J Pathol Microbiol. 2007; 50 (4): 730-2.
Chhabra S, Minz RW, Saikia B. Immunofluorescence in dermatology. Indian J Dermatol Venereol Leprol 2012;78:677‑91
Skiljević D. [Diagnostic significance of immunofluorescent tests in dermatology].Med Pregl. 2009 Nov-Dec;62(11-12):539-46.Am J Dermatopathol. 2016 Apr;38(4):283-8.
Elenitsas R, Nousari CH, Seykora JT. Laboratory methods. In: Elder DE, Elenitsas R, Johnson BL, Murphy GF, editors. Lever’s Histopathology of the Skin. 9th ed. Philadelphia: JB Lippincott; 2005. p. 59‑73.
Weedon D, editor. The vesiculobullous reaction pattern. In: Weedon’s Skin Pathology. 3rd ed. China: Churchill Livingstone Elsevier; 2010.p. 124‑68.
Buch C A , Kumar Harsh, NK Panicker, Misal Sonali, YK Sharma .;A Cross-sectional Study of Direct Immunofluorescence in the Diagnosis of Immunobullous Dermatoses. Indian J Dermatol. 2014 Jul-Aug; 59(4): 364–68.
Charisma Kunhi Khannan, Ramesh Bhat M. A retrospective study of clinical, histopathological and direct immunofluorescence spectrum of immunobullous Disorders. International Journal of Scientific and Research Publications, Volume 5, Issue 9, September 2015.
Nageswaramma.S , Vani.T, Swarnakumari.G , Ram mohan .Ch, Sirisha.G, Sowjanya.p, Direct Immmuno Flourescence Study in Auto Immune Bullous Disorders, IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) e-ISSN: 2279-0853, p-ISSN: 2279-0861.Volume 14, Issue 1 Ver. VI (Jan. 2015), PP 06-08
Kambil M Srinath, P Madavamurthy, Immunobullous disorders: Clinical histopathological and immunofluorescence study of thirty-six cases, Year : 2014 | Volume : 5 | Issue : 2 | Page : 134-138.
Shamim T1, Varghese VI, Shameena PM, Sudha S., Pemphigus vulgaris in oral cavity: clinical analysis of 71 cases. Med Oral Patol Oral Cir Bucal. 2008 Oct 1;13 (10):E622-6.
Joe West, PhD,1,2,* Kate M Fleming, PhD,1 Laila J Tata, PhD,1 Timothy R Card, PhD,1,2 and Colin J Crooks, PhD. Incidence and Prevalence of Celiac Disease and Dermatitis Herpetiformis in the UK Over Two Decades: Population-Based Study.
Arya SR, Valand AG, Krishna K., A Clinico-pathological study of 70 cases of pemphigus. Indian J Dermatol Venereol Leprol 1999; 168-71.
P.Deepti*, M.S.Sulakshana, Y.A. Manjunatha and H.T. Jayaprakash, A histomorphological study of bullous lesions of skin with special reference to immunofluorescence international journal of current research and academic review, ISSN: 2347-3215 Volume 3 Number 3 (March-2015) pp. 29-51
Ueki H, Yaoita H, eds. A Color Atlas of Dermato-immunohistocytology. 1st ed. Tokyo: Wolfe Medical Publications; 1989.
Narsimha Rao Netha G1*, Geeta Kiran A1, Tulasi J,Merugu Ramchander 2 and Sambaru Kalyani2, Direct Immunofluorescence in Immunobullous disorders: A case study , Current Research in Medicine and Medical Sciences, accepted 04 December 2014
Vedartham Kishore Kumar, A Study of Direct Immunofluorescence in the diagnosis of Immunobullous Dermatoses, DOI: 10.18410/jebmh/2015/617
Roseli Svartman Isfer et al. Direct immunofluorescence in Lupus Erythematosus (LE), Sao Paulo Med. J. vol.114 no.2: 1141-47
Lebe B, Gül Nýflýoðlu G, Seyrek S, Ellýdokuz H. Evaluation of clinical and histopathologic/direct immunofluorescence diagnosis in autoimmune vesiculobullous dermatitis: Utility of direct immunofluorescence. Turk Patoloji Derg 2012;28:11-6.
