Effects of L-Carnitine supplementation on relative gene expression of OCTN1, OCTN2, OCTN3 in mitochondria and skin in meldonium induced carnitine depleted male albino Wistar Rats
Keywords:
Organic cation transporter OCTN protein, Rat, Gene expression, impaired glucose metabolismAbstract
Introduction L-carnitine supplementation may have a positive impact on the expression of genes involved in fatty acid metabolism, including the OCTN genes that are responsible for L-carnitine uptake into cells. However, further research is needed to fully understand the molecular mechanisms underlying these effects and their implications for human health. Methodology A total number of n=48 Albino Wistar rats were recruited and divided into four groups 12rats in each group. Group A was kept control whereas animals in remaining three groups were administered meldonium mixed with food in the form of powder. Meldonium was administered at 100mg/kg of body weight for the purpose of L-carnitine depletion for 10 days. Results Relative OCTN 1 gene expression in group A was noted as 1.2 ± 0.01 in contrast to the experimental groups B 2.1 ± 0.1, C 4.6 ± 0.7, D 6.2 ± 0.8 and E as 8.0 ± 0.9. Low and high doses of L-carnitine upregulated the expression of OCTN2 gene (P= 0.0001) Conclusion Overall, findings suggest that L-carnitine supplementation may have a positive impact on the expression of genes involved in fatty acid metabolism, including the OCTN genes that are responsible for L-carnitine uptake into cellsReferences
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