Effects of L-Carnitine supplementation on relative gene expression of OCTN1, OCTN2, OCTN3 in mitochondria and skin in meldonium induced carnitine depleted male albino Wistar Rats
DOI:
https://doi.org/10.66344/jpad.33.4.2023.2368Keywords:
Organic cation transporter OCTN protein, Rat, Gene expression, impaired glucose metabolismAbstract
Introduction L-carnitine supplementation may have a positive impact on the expression of genes involved in fatty acid metabolism, including the OCTN genes that are responsible for L-carnitine uptake into cells. However, further research is needed to fully understand the molecular mechanisms underlying these effects and their implications for human health. Methodology A total number of n=48 Albino Wistar rats were recruited and divided into four groups 12rats in each group. Group A was kept control whereas animals in remaining three groups were administered meldonium mixed with food in the form of powder. Meldonium was administered at 100mg/kg of body weight for the purpose of L-carnitine depletion for 10 days. Results Relative OCTN 1 gene expression in group A was noted as 1.2 ± 0.01 in contrast to the experimental groups B 2.1 ± 0.1, C 4.6 ± 0.7, D 6.2 ± 0.8 and E as 8.0 ± 0.9. Low and high doses of L-carnitine upregulated the expression of OCTN2 gene (P= 0.0001) Conclusion Overall, findings suggest that L-carnitine supplementation may have a positive impact on the expression of genes involved in fatty acid metabolism, including the OCTN genes that are responsible for L-carnitine uptake into cellsReferences
Shahouzehi B, Masoumi-Ardakani Y, Fallah H, Aminizadeh S. Concomitant Administration of L-carnitine and Performing High-Intensity Interval Training Effects on the Genes Involved in Mitochondrial Fusion and Apoptosis in Rat Liver. Jordan Journal of Biological Sciences. 2023 Mar;16(1).
Koepsell H. Organic cation transporters in health and disease. Pharmacological reviews. 2020 Jan 1;72(1):253-319.
Koleske ML, McInnes G, Brown JE, Thomas N, Hutchinson K, Chin MY, Koehl A, Arkin MR, Schlessinger A, Gallagher RC, Song YS. Functional genomics of OCTN2 variants informs protein-specific variant effect predictor for Carnitine Transporter Deficiency. 2022 Nov 15;119(46):e2210247119.
Amiri R, Tabandeh MR, Hosseini SA. Novel cardioprotective effect of L-carnitine on obese diabetic mice: Regulation of chemerin and CMKLRI expression in heart and adipose tissues. Arquivos Brasileiros de Cardiologia. 2021 Oct 25;117:715-25.
Jing Z, Okubo H, Morishige JI, Xu P, Hasan N, Nagata N, Ando H. Lenvatinib causes reduced expression of carnitine/organic cation transporter 2 and carnitine deficiency in the skeletal muscle of rats. Toxicology Letters. 2022 Aug 1;366:17-25.
Li J, Liu L, Weng J, Yin TL, Yang J, Feng HL. Biological roles of lâ€carnitine in oocyte and early embryo development. Molec
ular Reproduction and Development. 2021 Oct;88(10):673-85.
Gyawali A, Hyeon SJ, Ryu H, Kang YS. The alteration of l-carnitine transport and pretreatment effect under glutamate cytotoxicity on motor neuron-like NSC-34 lines. Pharmaceutics. 2021 Apr 14;13(4):551.
Dudicova S, Kacerovsky M, Ceckova M. Effect of inflammatory environment on the gene expression of OCTN1 (SLC22A4) and OCTN2 (SLC22A5) transporters in human trophoblast cell lines BeWo, HTR-8/SVneo and placental villous explants.
Jennaro TS, Puskarich MA, McCann MR, Gillies CE, Pai MP, Karnovsky A, Evans CR, Jones AE, Stringer KA. Using lâ€Carnitine as a Pharmacologic Probe of the Interpatient and Metabolic Variability of Sepsis. Pharmacotherapy: The Journal of Human Pharmacology and Drug Therapy. 2020 Sep;40(9):913-23.
Alhasaniah AH. l-carnitine: Nutrition, pathology, and health benefits. Saudi Journal of Biological Sciences. 2022 Dec 30:103555.
Pavelka K, Bruyere O, Cooper C, Kanis JA, Leeb BF, Maheu E, Martel-Pelletier J, Monfort J, Pelletier JP, Rizzoli R, Reginster JY. Diacerein: benefits, risks and place in the management of osteoarthritis. An opinion-based report from the ESCEO. Drugs & aging. 2016 Feb;33:75-85.
Lamhonwah AM, Tein I. Expression of the organic cation/carnitine transporter family (Octn1,-2 and-3) in mdx muscle and heart: Implications for early carnitine therapy in Duchenne muscular dystrophy to improve cellular carnitine homeostasis. Clinica Chimica Acta. 2020 Jun 1;505:92-7.
Kwon KC, Won JG, Myoung J, Kim MS, Cho Y, Park SW, Song YS. A Study on Evaluation of Exfoliation Efficacy and Erythema Relief by Carnitine Salicylate Ion Pair System. Korean Journal of Cosmetic Science. 2021;3(1):1-2.
Downloads
Published
How to Cite
Issue
Section
License
Submission declaration
Authors retain the copyright to their work and grant the 'Journal of Pakistan Association of Dermatologists (JPAD)' the right of first publication under a Creative Commons Attribution 4.0 International (CC BY 4.0) license. This license allows others to share, adapt, and reuse the work for any purpose, including commercial use, as long as appropriate credit is given to the original authors and the journal.
By submitting a manuscript, authors confirm that the work has not been published previously (except as an abstract, lecture, or academic thesis), is not under review elsewhere, and has been approved by all authors and relevant authorities. Once accepted, the article will be openly accessible under the CC BY 4.0 license, ensuring wide dissemination and reuse with proper attribution.
