A survey on Helicobacter pylori seropositivity status in Iranian children with atopic dermatitis
Keywords:
Atopic dermatitis, Helicobacter pylori, childrenAbstract
Objective To evaluate the presence of anti-Helicobacter pylori antibody in the sera of children with and without atopic dermatitis (AD) in Kerman province, Iran. Patients and methods In the current study the cases consisted of 128 patients with AD, aged from 6 month to 16 years. One hundred and twenty eight healthy children matched for sex and age were chosen as control subjects. The enrolled patients were selected consequentially from the cases referred to the dermatology and pediatric clinics of Afzalipour hospital, an academic medical referral center in the capital of Kerman province. The diagnosis of AD was made based on the UK working party’s diagnostic criteria for atopic dermatitis. The control subjects were chosen randomly among children who were referred to the center for periodic vaccination program and before receiving the vaccines. The subjects in control group who had a personal or family history of allergies (including asthma, atopic dermatitis or allergic rhinitis) were excluded as well. A sample consisting of 3 milliliters of blood was obtained from each subject in the study (including the cases and the controls) for H. pylori serology. In children under 1 year of age, the amount of collected blood samples we reduced to the minimum in the acceptable range. The status of infection with Helicobacter pylori was determined in all subjects quantitatively by measurement of specific IgG antibody via enzyme-linked immunosorbent assay (ELISA) method (Trinity Biotech Monbind CaptiaTM, USA). Results The mean age of the cases with AD and the controls were 5.74± 4.05 and 6.05±3.36 years, respectively. Of all the children in both cases and controls 45.5 % and 55.8 % were females, respectively and the remaining were males. Anti-H. pylori IgG antibody was positive in the cases with AD and the control group 25.2 % and 24%, respectively that was not statistically significant. Conclusion The results of this study showed no association between H. pylori infection and AD.References
Kjellman NIM, Nilson L. From food allergy and atopic dermatitis to respiratory allergy. Pediatr Allergy Immunol. 1998;9 (Supple. 11):13-7.
Bursh AW, James JM, Hiegel A et al. Atopic dermatitis and food hypersensitivity reactions. J Pediatr. 1998:132:132-6.
Chandra RK, Gill B, Kumari S. Food allergy and atopic disease: Pathogenesis, diagnosis, prediction of high risk, and prevention. Ann Allergy. 1993;71:495-502.
Hanifin JM, Rogge JL. Staphylococcal infections in patients with atopic dermatitis. Arch Dermatol. 1977;113:1383-6.
Sampson HA, Scanlon SM. Natural history of food hypersensitivity in children with atopic dermatitis. J Pediatr. 1989;115:23-7.
Corrado G, Luzzi I, Pacchiarotti C et al. Helicobacter pylori seropositivity in children with atopic dermatitis as sole manifestation of food allergy. Pediatr Allergy Immunol. 2000:11:101-5.
Lopez Perez G, Alcantara F. Relation between Helicobacter pylori and atopic diseases. Rev Alerg Mex. 2002;29:157-62.
Sakurane M, Shiotani A, Furukawa F. Therapeutic effects of antibacterial treatment for intractable skin diseases in Helicobacter pylori-positive Japanese patients. J Dermatol. 2002;29:23-7.
Wedi B, Kapp A. Helicobacter pylori infection in skin diseases: a critical appraisal. Am J Clin Dermatol. 2002;3:273-82.
Mccune A, Lane A, Murray L et al. Reduced risk of atopic disorders in adults with Helicobacter pylori infection. Eur J Gastroenterol Hepatol. 2003;15:637-40.
Cremonini F, Gasbarrini A. Atopy, Helicobacter pylori and the hygiene hypothesis Eur J Gastroenterol Hepatol. 2003;15:635- 6.
Linneberg A, Ostergaard C, Tvede M et al. IgG antibodies against microorganisms and atopic disease in Danish adults. The Copenhagen Allergy study. J Allergy Clin lmmunol. 2003;111:847-53.
Everhart JE. Recent developments in the epidemiology of Helicobacter pylori. Gastroenterol Clin North Am. 2000;29:559-79.
Campbell DL, Thomas JE. H. pylori infection in pediatric practice. Arch Dis Child Edu Pract Ed. 2005;90:25-30.
Working Party of the European Helicobacter pylori. Study Group Guidelines for clinical trials in Helicobacter pylori infection. Gut. 1997;41 (Supple 2):S1-S9.
Pelser HH, Househam KC, Joubert J et al. Prevalence of Helicobacter pylori antibodies in children in Bloem fontein, South Africa. J Pediatr Gastroenterol Nutr. 1997;25:135-9.
Thomas JE, Whatmore AM, Barer MR et al. Serodiagnosis of Helicobacter pylori infection in childhood. J Clin Microbiol. 1990;28:2641-6.
McColl KEL. Helicobacter pylori infection. N Engl J Med. 2010;362:1597-1604.
Williams HC, Burney PGJ, Pembroke AC, Hay RJ. The UK working party’s diagnostic criteria for atopic dermatitis. III. Independent hospital validation. Br J Dermatol. 1994;131:406-16.
Valenta R, Seibeler S, Natter S et al. Autoallergy; A pathogenetic factor in atopic dermatitis? J Allergy Clin Immunol. 2000;105:432-7.
Bartuzi Z, Korenkiewicz J, Romański B. Correlation between Helicobacter pylori infection and food allergy in chronic gastritis. Med Sci Monit. 2000;6:530-8.
Murakami K, Fujioka T, Nishizono A et al. Atopic dermatitis successfully treated by eradication of Helicobacter pylori. J Gastroenterol. 1996;31 (supple. 9):77-82.
