Wistar Rat as Photoaging Mouse Model

Authors

  • Damayanti Doctoral Study Program of Medical Science, Faculty of Medicine Universitas Airlangga, Surabaya, Indonesia; Department of Dermatology and Venerology, Faculty of Medicine Universitas Airlangga/Dr. Soetomo Hospital, Surabaya, Indonesia
  • Cita Rosita Sigit Prakoeswa Department of Dermatology and Venerology, Faculty of Medicine Universitas Airlangga/Dr. Soetomo Hospital, Surabaya, Indonesia
  • Djoko Agus Purwanto Department of Pharmaceutical Chemistry, Faculty of Pharmacy Universitas Airlangga, Surabaya, Indonesia
  • Anang Endaryanto Department of Pediatrics, Faculty of Medicine Universitas Airlangga, Surabaya, Indonesia
  • Muhammad Yulianto Listiawan Department of Dermatology and Venerology, Faculty of Medicine Universitas Airlangga/Dr. Soetomo Hospital, Surabaya, Indonesia
  • Yohanes Widodo Wirohadidjoyo Department of Dermatology and Venerology, Faculty of Medicine Universitas Gadjah Mada, Yogyakarta, Indonesia
  • Soetjipto Department of Biochemistry, Faculty of Medicine Universitas Airlangga, Surabaya, Indonesia
  • Siswandono Department of Pharmaceutical Chemistry, Faculty of Pharmacy Universitas Airlangga, Surabaya, Indonesia
  • Budi Utomo Department of Community Medicine, Faculty of Medicine Universitas Airlangga, Surabaya, Indonesia

Keywords:

Photoaging, mouse model, wistar rat, life expectancy

Abstract

Background The increasing of human life expectancy may cause aging problems, including photoaging. Photoaging raises new problems in community, because its influence in human self-esteem and quality of life. Studies about photoaging have been extensively performed to enhance the basic mechanism of photoaging. Mouse skin is usually used as cutaneous aging model, because it is genetically similar to human skin.   Methods This study was true experimental study, post test only-control group design that was aimed to evaluate the role of ultraviolet (UV) B irradiation to produce photoaging mouse model. Twenty-four Wistar rats were divided randomly allocation into 2 groups, namely non-UV irradiated group and UV irradiated group. Ultraviolet B irradiation were performed for 5 weeks until reaching the total dose 3100 mJ/cm2. Dermal collagen density and transepidermal water loss (TEWL) level were evaluated after the intervention.   Results Dermal collagen density in non-UV irradiated group and UV irradiated group were 38.21±2.67% and 27.73±0.77%. TEWL level in non-UV irradiated group and UV irradiated group were 7.55±0.89 g/m2/h and 17.53±3.94 g/m2/h. There was significant difference between the groups in dermal collagen density and TEWL level (p<0.05).   Conclusion Wistar rat in UV irradiated group that were given total dose 3100 mJ/cm2 of UV B for 5 weeks has been proven to become photoaging mouse model.  

References

Puizina IN. Skin aging. Acta Dermatovenereol Alp Pannonica Adriat. 2008;17(2l):47-54.

Yaar M, Gilchrest BA. Aging of the skin. Fitzpatrick’s in General Medicine 8th ed., New York: The McGraw Hill Companies; 2012. p 1213-26.

Nabila YA, Damayanti, Handayani H, Setyaningrum T. The Effect of Lifestyle on Skin Aging. Berkala Ilmu Kesehatan Kulit dan Kelamin. 2021;33(2):110-5.

Hwang KA, Yi BR, Choi KC. Molecular mechanism and in vivo mouse models of skin aging associated with dermal matrix alterations. Lab Anim Res. 2011;27(1):1-8.

Gonzaga ER. Role of UV light in photodamage, skin aging, and skin cancer. Am J Clin Dermatol. 2009;10(1):19-24.

Lephart ED. Skin aging and oxidative stress: equol’s anti-aging effects via biochemical and molecular mechanism. Ageing Res Rev. 2016;31:36-54.

Ahmad Z, Damayanti. Skin Aging: Pathophysiology and Clinical Manifestation. Berkala Ilmu Kesehatan Kulit dan Kelamin. 2018;30(3):208-15.

Spencer TS. Transepidermal water loss. Methods for cutaneous investigation, New York: Marcell Dekker; 1990. p 191-217.

Schaefer H, Redelmeir TE, Nohynek GJ, Lademann J. Pharmacokinetics and topical applications of drugs. Fitzpatrick’s in General Medicine 8th ed., New York: The McGraw Hill Companies; 2012. p 2652-8.

Agustyniak AM, Rotsztejn H, Bartnicka E, Budzisz. Effects of reactive oxygen species on skin photoaging. Przegl Dermatol. 2016;103:233-9.

Chang KCN, Wang Y, Oh IG, Jenkins S, Freedman LP, Thompson CC et al. Esterogen receptor β is a novel therapeutic target for photoaging. Mol Pharmacol. 2010;77(5):744-50.

Kim YJ, Kim HN, Shin MS, Choi BY. Thread embedding acupuncture inhibits ultraviolet B irradiation-induced skin photoaging in hairless mice. Evid Based Complement Alternat Med. 2015;1-9.

doi: 10.1115/2015/539172.

Kwon S, Jung SH. Topical administration of manuka oil prevents UV-B irradiation-induced cutaneous photoaging in mice. Evid Based Complement Alternat Med. 2013;2013:1-10.

doi: 10.1155/2013/930857.

Chen Y, Yu Q, Bao Xu C. A convenient method for quantifying collagen fibers in atherosclerotic lesions by ImageJ software. Int J Clin Exp Med. 2017;10(10):14904-10.

Choi SI, Jung TD, Cho BY, Choi SH, Sim WS, Han X, et al. Anti-photoaging effect of fermented agricultural by-products on ultraviolet B-irradiated hairless mouse skin. Int J Mol Med. 2019;44:559-68.

Plessis J, Stefaniak A, Eloff F, John S, Agner T, Chou TC, et al. International guidelines for the in vivo assessment of skin properties in non-clinical settings: part 2. transepidermal water loss and skin hydration. Skin Res Technol. 2013;19(3):265-78.

Gardien KLM, Baas DC, Vet HCW, Middelkoop E. Transepidermal water loss measured with the Tewameter TM300 in burn scar. Burns. 2016;30:1-8.

Rittie L, Fisher GJ. UV-light-induced signal cascades and skin aging. Ageing Res Rev. 2002;1:705-20.

Chiu HW, Chen CH, Chen YJ, Hsu YH. Far infrared suppresses in skin photoaging in ultraviolet B-exposed fibroblast and hairless mice. PLoS One. 2017;12(3):1-15.

Poon F, Kang S, Chien AL. Mechanism and treatments of photoaging. Photodermatol Photoimmunol Photomed. 2014;31:65-74.

Pittayapruek P, Meephasan J, Prapapan O, Komine M, Ohtsuki M. Role of matrix metalloproteinases in photoaging and photocarcinogenesis. Int J Mol Sci. 2016;17:868(1-20).

Dong KK, Damaghi N, Picart SD, Markova NG, Obayashi K, Okano Y, et al. UV-induced DNA damage initiates release of MMP-1 in human skin. Exp Dermatol. 2008;17:1037-44.

Hung CF, Chen WY, Aljuffali IA, Lin YK, Shih HC, Fang JY. Skin aging modulates percutaneous drug absorbtion: the impact of ultaviolet irradiation and ovariectomy. Age. 2015;37(21):1-14.

Lee KO, Kim SN, Kim YC. Anti-wrinkle effect of water extracts of teas in hairless mouse. Toxicol Res. 2014;30(4):283-9.

Downloads

Published

2023-03-05

How to Cite

1.
Damayanti, Prakoeswa CRS, Purwanto DA, Endaryanto A, Listiawan MY, Wirohadidjoyo YW, Soetjipto, Siswandono, Utomo B. Wistar Rat as Photoaging Mouse Model. J Pak Assoc Dermatol [Internet]. 2023Mar.5 [cited 2025Jan.14];33(1):24-9. Available from: http://www.jpad.com.pk/index.php/jpad/article/view/2016

Issue

Vol. 33 No. 1 (2023): January-March

Section

Original Articles

Most read articles by the same author(s)

1 2 3 > >>